Hotspot

trnP-UGG-psaJ

LSC · 66869-67346 · 478 bp

Back to Breynia

Pi 0.0153

MarkerSeek score 66.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 13
pi: 0.0153
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	549-608 bp	1.0	83.7
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	548-607 bp	1.0	83.7
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	560-619 bp	1.0	83.6
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	559-618 bp	1.0	83.6
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	CGGTGCCACGGATAGATATG	50.0	55.0	58.1	58.0	562-621 bp	1.0	83.5