Hotspot

petA-psbJ

LSC · 63861-64786 · 926 bp

Back to Breynia

Pi 0.0162

MarkerSeek score 69.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 926
variable_sites: 24
pi: 0.0162
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CATTACGTGTCCAAGGCCTT	ACTGGTATTCCTGTGATCGG	50.0	50.0	58.2	56.7	1117-1137 bp	1.0	70.2
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	CTGGTATTCCTGTGATCGGT	50.0	50.0	58.2	56.7	1116-1136 bp	1.0	70.2
petA-psbJ_p3	GACCCATTACGTGTCCAAGG	ACTGGTATTCCTGTGATCGG	55.0	50.0	58.3	56.7	1121-1141 bp	1.0	69.5
petA-psbJ_p4	GACCCATTACGTGTCCAAGG	CTGGTATTCCTGTGATCGGT	55.0	50.0	58.3	56.7	1120-1140 bp	1.0	69.5
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	CTGGTATTCCTGTGATCGGT	45.0	50.0	57.6	56.7	1114-1134 bp	1.0	68.8