Hotspot

rbcL-accD

LSC · 57203-57994 · 792 bp

Back to Breynia

Pi 0.0162
MarkerSeek score 61.40
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
792
variable_sites
22
pi
0.0162
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGGCTAGCAAATGGAGTCC AACATCGAATTGAACCACCT 55.0 40.0 58.0 55.3 904-915 bp 1.0 63.6
rbcL-accD_p2 GAGGCTAGCAAATGGAGTCC ACATCGAATTGAACCACCTT 55.0 40.0 58.0 55.3 903-914 bp 1.0 63.6
rbcL-accD_p3 GAGGCTAGCAAATGGAGTCC AACATCGAATTGAACCACCTT 55.0 38.1 58.0 56.1 904-915 bp 1.0 62.1
rbcL-accD_p4 GAGGCTAGCAAATGGAGTCC ACATCGAATTGAACCACCTTT 55.0 38.1 58.0 56.1 903-914 bp 1.0 62.1
rbcL-accD_p5 GAGTCCTGAACTAGCTGCTG ACATCGAATTGAACCACCTT 55.0 40.0 57.7 55.3 889-900 bp 1.0 61.6