Hotspot

ndhC-trnM-CAU

LSC · 50666-52750 · 2085 bp

Back to Breynia

Pi 0.0107
MarkerSeek score 66.70
Primer available yes
Label ndhC-trnM-CAU

Feature Metadata

label_name
ndhC-trnM-CAU
feature_type
igs
region
LSC
length_bp
2085
variable_sites
38
pi
0.0107
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAU_p1 CCCTTTGCTAGTTGGAGCTA TTGAACCAATGACTCCTGCC 50.0 50.0 56.9 58.1 2254-2342 bp 1.0 73.4
ndhC-trnM-CAU_p2 GGCCCTTTGCTAGTTGGAG TTGAACCAATGACTCCTGCC 57.9 50.0 58.1 58.1 2256-2344 bp 1.0 73.0
ndhC-trnM-CAU_p3 CCCTTTGCTAGTTGGAGCTA CTCCTGCCGTATGAAAGCAA 50.0 50.0 56.9 58.3 2242-2330 bp 1.0 72.0
ndhC-trnM-CAU_p4 GGCCCTTTGCTAGTTGGAG CTCCTGCCGTATGAAAGCAA 57.9 50.0 58.1 58.3 2244-2332 bp 1.0 71.6
ndhC-trnM-CAU_p5 CCCTTTGCTAGTTGGAGCTAA TTGAACCAATGACTCCTGCC 47.6 50.0 57.6 58.1 2254-2342 bp 1.0 71.2