Hotspot

trnR-UCU-atpA

LSC · 10361-10635 · 275 bp

Back to Breynia

Pi 0.0418

MarkerSeek score 61.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 275
variable_sites: 16
pi: 0.0418
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAAAAGCGTCCATTGTCT	AGGAACAGCTGGAACGTTTT	45.0	45.0	57.7	57.9	372-444 bp	1.0	84.6
trnR-UCU-atpA_p2	TGGAAAAGCGTCCATTGTCT	ATTCAGGAACAGCTGGAACG	45.0	50.0	57.7	58.2	376-448 bp	1.0	83.5
trnR-UCU-atpA_p3	TGGAAAAGCGTCCATTGTCT	GGAACAGCTGGAACGTTTTC	45.0	50.0	57.7	57.6	371-443 bp	1.0	81.8
trnR-UCU-atpA_p4	TGGAAAAGCGTCCATTGTCT	CAGGAACAGCTGGAACGTTT	45.0	50.0	57.7	58.7	373-445 bp	1.0	79.5
trnR-UCU-atpA_p5	TGGAAAAGCGTCCATTGTCT	CAGGAACAGCTGGAACGTT	45.0	52.6	57.7	58.0	373-445 bp	1.0	77.0