Hotspot

trnS-GCU-trnR-UCU

LSC · 8555-10288 · 1734 bp

Back to Breynia

Pi 0.00533

MarkerSeek score 47.20

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1734
variable_sites: 18
pi: 0.00533
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1805-1945 bp	1.0	65.9
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1804-1944 bp	1.0	65.9
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1811-1951 bp	1.0	65.3
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1810-1950 bp	1.0	65.3
trnS-GCU-trnR-UCU_p5	TCGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.9	56.9	1859-1999 bp	1.0	64.8