Hotspot

matK-trnK-UUU

LSC · 3507-4243 · 737 bp

Back to Breynia

Pi 0.00663
MarkerSeek score 57.60
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
737
variable_sites
9
pi
0.00663
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 GACTCCCGAAAGATAAGTGGA AACGGTAGAGTACTCGGCTT 47.6 50.0 56.8 58.2 846-853 bp 1.0 62.9
matK-trnK-UUU_p2 GACTCCCGAAAGATAAGTGGA ACGGTAGAGTACTCGGCTTT 47.6 50.0 56.8 58.2 845-852 bp 1.0 62.9
matK-trnK-UUU_p3 AGACTCCCGAAAGATAAGTGG AACGGTAGAGTACTCGGCTT 47.6 50.0 56.8 58.2 847-854 bp 1.0 62.9
matK-trnK-UUU_p4 AGACTCCCGAAAGATAAGTGG ACGGTAGAGTACTCGGCTTT 47.6 50.0 56.8 58.2 846-853 bp 1.0 62.9
matK-trnK-UUU_p5 AGACTCCCGAAAGATAAGTGGA ACGGTAGAGTACTCGGCTTT 45.5 50.0 58.3 58.2 846-853 bp 1.0 61.9