Hotspot

psbA

LSC · 307-1368 · 1062 bp

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Pi 0.00246
MarkerSeek score 36.10
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
17
pi
0.00246
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 AAACTCGTCTAAATTCAGACT AGAATTCGTGTGCTTGGGAG 33.3 50.0 52.6 58.2 1147 bp 1.0 41.2
psbA_p2 AAACTCGTCTAAATTCAGACT AGAGAATTCGTGTGCTTGGG 33.3 50.0 52.6 58.2 1149 bp 1.0 41.2
psbA_p3 AAACTCGTCTAAATTCAGACT GAGAATTCGTGTGCTTGGGA 33.3 50.0 52.6 58.2 1148 bp 1.0 41.2
psbA_p4 AAACTCGTCTAAATTCAGACT TGGGAGTCCCTGATGATGAA 33.3 50.0 52.6 57.7 1133 bp 1.0 41.2
psbA_p5 AAACTCGTCTAAATTCAGACT TTGGGAGTCCCTGATGATGA 33.3 50.0 52.6 57.7 1134 bp 1.0 41.2