Hotspot

psaC

SSC · 118917-119162 · 246 bp

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Pi 0.00577
MarkerSeek score 26.90
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
5
pi
0.00577
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 ACGTTTCTGTCCTCCCCATA TTATAGCTATTGCCGCAGCC 50.0 50.0 58.1 58.2 1205-1229 bp 1.0 83.6
psaC_p2 TTTGACCGCTGTACATTGCT TTATAGCTATTGCCGCAGCC 45.0 50.0 58.1 58.2 1101-1125 bp 1.0 83.6
psaC_p3 ACGTTTCTGTCCTCCCCATA ATCTTAGCCAACCCAGGAGT 50.0 50.0 58.1 58.0 1015-1024 bp 0.925926 80.2
psaC_p4 TTTGACCGCTGTACATTGCT ATCTTAGCCAACCCAGGAGT 45.0 50.0 58.1 58.0 911-920 bp 0.925926 80.1
psaC_p5 ACCCCAAAACCCGTTCTTTT ATCTTAGCCAACCCAGGAGT 45.0 50.0 58.1 58.0 514-522 bp 0.62963 68.3