Hotspot

ccsA-psaC

SSC · 116943-118916 · 1974 bp

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Pi 0.0111
MarkerSeek score 53.10
Primer available yes
Label ccsA-psaC

Feature Metadata

label_name
ccsA-psaC
feature_type
igs
region
SSC
length_bp
1974
variable_sites
96
pi
0.0111
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-psaC_p1 ATGCAGCTCTTTTATGCGGA AAACAACTCGAAGCATGGGT 45.0 45.0 58.0 58.0 2464-2521 bp 1.0 85.1
ccsA-psaC_p2 TGCAGCTCTTTTATGCGGAT AAACAACTCGAAGCATGGGT 45.0 45.0 58.0 58.0 2463-2520 bp 1.0 85.1
ccsA-psaC_p3 ATGCAGCTCTTTTATGCGGA CCTTGGGATGGATGTAAGGC 45.0 55.0 58.0 58.0 2591-2648 bp 1.0 85.0
ccsA-psaC_p4 TGCAGCTCTTTTATGCGGAT CCTTGGGATGGATGTAAGGC 45.0 55.0 58.0 58.0 2590-2647 bp 1.0 85.0
ccsA-psaC_p5 TGCAGCTCTTTTATGCGGAT CAAATTGCTTCTGCTCCACG 45.0 50.0 58.0 58.0 2566-2623 bp 1.0 84.9