Hotspot

rpl14-rps3

LSC · 82336-84069 · 1734 bp

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Pi 0.00691
MarkerSeek score 60.60
Primer available yes
Label rpl14-rps3

Feature Metadata

label_name
rpl14-rps3
feature_type
igs
region
LSC
length_bp
1734
variable_sites
69
pi
0.00691
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rps3_p1 CGCGACATTCAAATGGGTTT CTCTACAAACCATTCGGGCT 45.0 50.0 57.7 57.6 1833-1874 bp 1.0 79.4
rpl14-rps3_p2 CGCGACATTCAAATGGGTTT CAGTCCGAACGATCTATGGG 45.0 55.0 57.7 57.6 1791-1832 bp 1.0 79.3
rpl14-rps3_p3 CGCGACATTCAAATGGGTTT CCTCTACAAACCATTCGGGC 45.0 55.0 57.7 58.6 1834-1875 bp 1.0 77.6
rpl14-rps3_p4 CACATCAATTTTCGAGCCCC CTCTACAAACCATTCGGGCT 50.0 50.0 57.4 57.6 1862-1903 bp 1.0 77.5
rpl14-rps3_p5 CACATCAATTTTCGAGCCCC CAGTCCGAACGATCTATGGG 50.0 55.0 57.4 57.6 1820-1861 bp 1.0 77.5