Hotspot

trnE-UUC-trnT-GGU

LSC · 31442-32419 · 978 bp

Back to Bredia

Pi 0.00938
MarkerSeek score 62.30
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
978
variable_sites
56
pi
0.00938
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 1064-1101 bp 1.0 85.9
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 1061-1098 bp 1.0 85.8
trnE-UUC-trnT-GGU_p3 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.7 58.1 1005-1042 bp 1.0 85.4
trnE-UUC-trnT-GGU_p4 TGTCCTGAACCACTAGACGA TGGCGTTACTCTACCACTGA 50.0 50.0 57.7 58.1 1002-1039 bp 1.0 85.2
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC ACCATGGCGTTACTCTACCA 55.0 50.0 57.8 58.4 1065-1102 bp 1.0 82.9