Hotspot

petN-psbM

LSC · 29480-29842 · 363 bp

Back to Bredia

Pi 0.0195
MarkerSeek score 67.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
363
variable_sites
30
pi
0.0195
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA AAACCGTGAGTCAAGGTGAT 45.0 45.0 58.0 57.1 441-471 bp 1.0 82.1
petN-psbM_p2 TCTTGCTTGGGCTGCTTTAA AACCGTGAGTCAAGGTGATT 45.0 45.0 58.0 57.1 440-470 bp 1.0 82.1
petN-psbM_p3 TCTTGCTTGGGCTGCTTTAA AAAACCGTGAGTCAAGGTGA 45.0 45.0 58.0 57.3 463 bp 0.037037 35.8
petN-psbM_p4 AGTAAGTCTTGCTTGGGCTG AAAACCGTGAGTCAAGGTGA 50.0 45.0 57.8 57.3 469 bp 0.037037 34.3
petN-psbM_p5 GGCTGCTTTAATGGTCGTCT AAAACCGTGAGTCAAGGTGA 50.0 45.0 58.3 57.3 454 bp 0.037037 33.8