Hotspot

psbK-psbI

LSC · 7296-7668 · 373 bp

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Pi 0.0138
MarkerSeek score 64.00
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
373
variable_sites
32
pi
0.0138
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG TTTTTATTCGTCACGCCCGG 50.0 50.0 57.9 59.2 512-527 bp 1.0 79.3
psbK-psbI_p2 TGGCAAGCTGCTGTAAGTTT TTTTTATTCGTCACGCCCGG 45.0 50.0 58.3 59.2 509-524 bp 1.0 77.3
psbK-psbI_p3 TTGGCAAGCTGCTGTAAGTT TTTTTATTCGTCACGCCCGG 45.0 50.0 58.3 59.2 510-525 bp 1.0 77.3
psbK-psbI_p4 TTTGGCAAGCTGCTGTAAGT TTTTTATTCGTCACGCCCGG 45.0 50.0 58.3 59.2 511-526 bp 1.0 77.3
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG GGGATTACGTCCTGGATCAT 50.0 50.0 57.9 56.5 494-509 bp 1.0 76.8