Hotspot

ycf1

SSC · 122920-128202 · 5283 bp

Pi 0.0148

MarkerSeek score 52.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5283
variable_sites: 192
pi: 0.0148
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCGTCCCCCAAACAGTTAAA	GGATCTCTCGCGGAAGAATC	50.0	55.0	58.0	57.9	1200-1209 bp	1.0	84.5
ycf1_p2	CCGTCCCCCAAACAGTTAAA	CTGGAAGAATTGGAAGGCGA	50.0	50.0	58.0	57.9	838-847 bp	0.666667	70.3
ycf1_p3	CCGTCCCCCAAACAGTTAAA	AAGGCGAAACAGACGAAACT	50.0	45.0	58.0	58.1	825-834 bp	0.5	61.7
ycf1_p4	CCGTCCCCCAAACAGTTAAA	GACGAAACTGTACCAACGGA	50.0	50.0	58.0	57.9	814-823 bp	0.5	61.0
ycf1_p5	CCGTCCCCCAAACAGTTAAA	TGGCTTTGATCCGTTATGCA	50.0	45.0	58.0	57.9	684-687 bp	0.333333	52.4