Hotspot

trnT-ACC-psbD

LSC · 30875-32091 · 1217 bp

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Pi 0.0184
MarkerSeek score 52.10
Primer available yes
Label trnT-ACC-psbD

Feature Metadata

label_name
trnT-ACC-psbD
feature_type
igs
region
LSC
length_bp
1217
variable_sites
57
pi
0.0184
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACC-psbD_p1 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1273-1304 bp 1.0 45.8
trnT-ACC-psbD_p2 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 55.0 40.0 58.1 53.0 1296-1327 bp 1.0 45.7
trnT-ACC-psbD_p3 GTGGTAGAGTAACGCCATGG TTACCAAGGGCTATAGTCAT 55.0 40.0 58.1 52.8 1295-1326 bp 1.0 45.7
trnT-ACC-psbD_p4 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.1 53.0 1299-1330 bp 1.0 45.6
trnT-ACC-psbD_p5 TCAGTGGTAGAGTAACGCCA TTACCAAGGGCTATAGTCAT 50.0 40.0 58.1 52.8 1298-1329 bp 1.0 45.6