Hotspot

trnE-GAA-trnT-ACC

LSC · 30270-30802 · 533 bp

Back to Braya

Pi 0.028
MarkerSeek score 69.40
Primer available yes
Label trnE-GAA-trnT-ACC

Feature Metadata

label_name
trnE-GAA-trnT-ACC
feature_type
igs
region
LSC
length_bp
533
variable_sites
34
pi
0.028
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-GAA-trnT-ACC_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 666-686 bp 1.0 86.5
trnE-GAA-trnT-ACC_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 645-665 bp 1.0 86.3
trnE-GAA-trnT-ACC_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 642-662 bp 1.0 86.1
trnE-GAA-trnT-ACC_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 628-648 bp 1.0 86.1
trnE-GAA-trnT-ACC_p5 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 607-627 bp 1.0 86.1