Hotspot

psbK-psbI

LSC · 7045-7359 · 315 bp

Back to Braya

Pi 0.0221
MarkerSeek score 60.40
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
315
variable_sites
16
pi
0.0221
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCAGCTGTAAG TTCTTCACGTCCCGGATTAC 50.0 50.0 57.9 57.3 445-457 bp 1.0 79.1
psbK-psbI_p2 AGCAGCTGTAAGTTTTCGCT TTCTTCACGTCCCGGATTAC 45.0 50.0 58.1 57.3 437-449 bp 1.0 78.7
psbK-psbI_p3 TGGCAAGCAGCTGTAAGTTT TTCTTCACGTCCCGGATTAC 45.0 50.0 58.3 57.3 442-454 bp 1.0 77.1
psbK-psbI_p4 TTGGCAAGCAGCTGTAAGTT TTCTTCACGTCCCGGATTAC 45.0 50.0 58.3 57.3 443-455 bp 1.0 77.1
psbK-psbI_p5 TTTGGCAAGCAGCTGTAAGT TTCTTCACGTCCCGGATTAC 45.0 50.0 58.3 57.3 444-456 bp 1.0 77.0