Hotspot

trnH-CAC-psbA

LSC · 77-393 · 317 bp

Back to Braya

Pi 0.0625
MarkerSeek score 82.60
Primer available yes
Label trnH-CAC-psbA

Feature Metadata

label_name
trnH-CAC-psbA
feature_type
igs
region
LSC
length_bp
317
variable_sites
40
pi
0.0625
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-CAC-psbA_p1 CTGCCTTAATCCACTTGGCT GTAACGCTCACAACTTCCCT 50.0 50.0 57.9 57.8 376-478 bp 1.0 89.1
trnH-CAC-psbA_p2 ACTGCCTTAATCCACTTGGC GTAACGCTCACAACTTCCCT 50.0 50.0 58.2 57.8 377-479 bp 1.0 88.9
trnH-CAC-psbA_p3 CTGCCTTAATCCACTTGGCT TGAACGTAACGCTCACAACT 50.0 45.0 57.9 57.8 381-483 bp 1.0 88.6
trnH-CAC-psbA_p4 ACTGCCTTAATCCACTTGGC TGAACGTAACGCTCACAACT 50.0 45.0 58.2 57.8 382-484 bp 1.0 88.4
trnH-CAC-psbA_p5 CTGCCTTAATCCACTTGGCT ATGCATGAACGTAACGCTCA 50.0 45.0 57.9 58.3 386-488 bp 1.0 88.0