Hotspot

rps15-trnN-GUU

SSC · 122828-128799 · 5972 bp

Back to Brassica

Pi 0.0117
MarkerSeek score 42.10
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
5972
variable_sites
273
pi
0.0117
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 TTTTTGTACAAGGATCCGAA GAACCTGTCCGTTTGTCTGT 35.0 50.0 52.7 58.1 736-771 bp 1.0 46.7
rps15-trnN-GUU_p2 TTGTACAAGGATCCGAATTT GAACCTGTCCGTTTGTCTGT 35.0 50.0 52.5 58.1 733-768 bp 1.0 46.7
rps15-trnN-GUU_p3 TTTGTACAAGGATCCGAATT GAACCTGTCCGTTTGTCTGT 35.0 50.0 52.5 58.1 734-769 bp 1.0 46.7
rps15-trnN-GUU_p4 TTTTTGTACAAGGATCCGAA TGAACCTGTCCGTTTGTCTG 35.0 50.0 52.7 58.1 737-772 bp 1.0 46.6
rps15-trnN-GUU_p5 TTTTTGTACAAGGATCCGAA TGTTGAACCTGTCCGTTTGT 35.0 45.0 52.7 57.9 740-775 bp 1.0 46.6