Hotspot

rpl20-rps12

LSC · 68068-68832 · 765 bp

Back to Brassica

Pi 0.0132

MarkerSeek score 47.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 39
pi: 0.0132
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	CTCAGCGACGAGGAACATG	50.0	57.9	58.0	58.3	801-836 bp	1.0	76.0
rpl20-rps12_p2	TTTTGTTCTACGCCTCCGAG	CTCAGCGACGAGGAACATG	50.0	57.9	57.9	58.3	815-850 bp	1.0	75.6
rpl20-rps12_p3	AGTTTTGTTCTACGCCTCCG	CTCAGCGACGAGGAACATG	50.0	57.9	58.2	58.3	817-852 bp	1.0	74.5
rpl20-rps12_p4	GTTTTGTTCTACGCCTCCGA	CTCAGCGACGAGGAACATG	50.0	57.9	58.2	58.3	816-851 bp	1.0	74.5
rpl20-rps12_p5	TTCTACGCCTCCGAGCTATA	CTCAGCGACGAGGAACATG	50.0	57.9	57.4	58.3	810-845 bp	1.0	71.8