Hotspot

psaJ-rpl33

LSC · 66262-66696 · 435 bp

Back to Brassica

Pi 0.0221

MarkerSeek score 54.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 435
variable_sites: 34
pi: 0.0221
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCAGATGCATTAACATTTCCCT	CGAACATCTTTACCTTTGGCC	39.1	47.6	58.1	57.5	476-502 bp	1.0	52.0
psaJ-rpl33_p2	TCCAGATGCATTAACATTTCCC	CGAACATCTTTACCTTTGGCC	40.9	47.6	56.7	57.5	476-502 bp	1.0	50.9
psaJ-rpl33_p3	CCAGATGCATTAACATTTCCCT	CGAACATCTTTACCTTTGGCC	40.9	47.6	56.7	57.5	475-501 bp	1.0	50.8
psaJ-rpl33_p4	TCCAGATGCATTAACATTTCCCT	CGAACACAACTGGTACATTCC	39.1	47.6	58.1	57.2	512-538 bp	1.0	48.9
psaJ-rpl33_p5	TCCAGATGCATTAACATTTCCC	CGAACACAACTGGTACATTCC	40.9	47.6	56.7	57.2	512-538 bp	1.0	48.4