Hotspot

ndhC-trnV-UAC

LSC · 49413-50279 · 867 bp

Back to Brassica

Pi 0.0631
MarkerSeek score 69.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
867
variable_sites
107
pi
0.0631
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.76
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAACAGGAATAGCACT GGTTCGAATCCGTATAGCCC 42.9 55.0 58.1 57.9 751-961 bp 1.0 82.4
ndhC-trnV-UAC_p2 CCCCGGAAATTAGAAATGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 56.8 57.9 767-977 bp 1.0 82.0
ndhC-trnV-UAC_p3 TGCCAAAACAGGAATAGCACT CAGGTCTACGGTTCGAATCC 42.9 55.0 58.1 57.8 760-970 bp 1.0 81.4
ndhC-trnV-UAC_p4 CCCCGGAAATTAGAAATGCC CAGGTCTACGGTTCGAATCC 50.0 55.0 56.8 57.8 776-986 bp 1.0 80.9
ndhC-trnV-UAC_p5 TGCCAAAACAGGAATAGCACT CGGTTCGAATCCGTATAGCC 42.9 55.0 58.1 58.3 752-962 bp 1.0 80.6