Hotspot

trnR-UCU-atpA

LSC · 9357-9574 · 218 bp

Back to Brassica

Pi 0.0252

MarkerSeek score 46.50

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 22
pi: 0.0252
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	GAGACCCAGTCAGGAGATGT	50.0	55.0	58.0	58.1	1038-1046 bp	1.0	81.9
trnR-UCU-atpA_p2	ACCCTTAGCCTTCCAAGCTA	GAGACCCAGTCAGGAGATGT	50.0	55.0	58.0	58.1	1044-1052 bp	1.0	81.9
trnR-UCU-atpA_p3	CGACGTCGACTAAAACCCTT	GAGACCCAGTCAGGAGATGT	50.0	55.0	57.9	58.1	1058-1066 bp	1.0	81.7
trnR-UCU-atpA_p4	AAACCCTTAGCCTTCCAAGC	GAGACCCAGTCAGGAGATGT	50.0	55.0	58.1	58.1	1046-1054 bp	1.0	81.6
trnR-UCU-atpA_p5	TTTGGGAATTTCGCCATCCA	GAGACCCAGTCAGGAGATGT	45.0	55.0	58.1	58.1	1156-1164 bp	1.0	81.4