Hotspot

trnK-UUU-rps16

LSC · 4230-4877 · 648 bp

Back to Brassica

Pi 0.0243
MarkerSeek score 54.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
648
variable_sites
60
pi
0.0243
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 967-1010 bp 1.0 88.9
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 968-1011 bp 1.0 88.9
trnK-UUU-rps16_p3 TCAGGATCAGTCGTGGTCTT CCCAATGAGCCGTTTATCGA 50.0 50.0 58.1 58.1 963-1006 bp 1.0 88.5
trnK-UUU-rps16_p4 TCGACACCGAAAACAAGGTT CCCAATGAGCCGTTTATCGA 45.0 50.0 58.0 58.1 1101-1102 bp 0.133333 44.8
trnK-UUU-rps16_p5 TCGACACCGAAAACAAGGTT TCCCAATGAGCCGTTTATCG 45.0 50.0 58.0 58.1 1102-1103 bp 0.133333 44.8