Hotspot

trnH-GUG-psbA

LSC · 76-258 · 183 bp

Back to Brassica

Pi 0.0645

MarkerSeek score 76.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 183
variable_sites: 40
pi: 0.0645
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	256-330 bp	1.0	90.4
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	259-333 bp	1.0	90.3
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	257-331 bp	1.0	90.2
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.2	57.9	260-334 bp	1.0	90.1
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.7	57.9	249-323 bp	1.0	88.8