Hotspot

atpF

LSC · 12871-14148 · 1278 bp

Back to Brassaiopsis

Pi 0.012

MarkerSeek score 54.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1278
variable_sites: 23
pi: 0.012
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	AGTCTATCTATAAGAGGAGATCA	50.0	34.8	57.7	52.5	1373-1374 bp	1.0	41.4
atpF_p2	ACTAATTTCGTCGGCTCGAA	AGTCTATCTATAAGAGGAGATCA	45.0	34.8	57.4	52.5	1385-1386 bp	1.0	41.4
atpF_p3	GGCTCGAATGGTTACCATGA	AGTCTATCTATAAGAGGAGATCAT	50.0	33.3	57.7	53.1	1373-1374 bp	1.0	41.4
atpF_p4	AATTTCGTCGGCTCGAATGG	AGTCTATCTATAAGAGGAGATCA	50.0	34.8	59.0	52.5	1382-1383 bp	1.0	41.4
atpF_p5	ACTAATTTCGTCGGCTCGAA	AGTCTATCTATAAGAGGAGATCAT	45.0	33.3	57.4	53.1	1385-1386 bp	1.0	41.4