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petA-psbJ

LSC · 65223-66251 · 1029 bp

Back to Brassaiopsis

Pi 0.0123

MarkerSeek score 66.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 19
pi: 0.0123
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.0	57.6	1174-1190 bp	1.0	78.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1157-1173 bp	1.0	77.1
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1171-1187 bp	1.0	76.9
petA-psbJ_p4	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1169-1185 bp	1.0	75.6
petA-psbJ_p5	TACGTGTCCAAGGCCTTTTG	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.4	57.6	1168-1184 bp	1.0	75.2