Hotspot

trnT-GGU-psbD

LSC · 33707-35072 · 1366 bp

Back to Brassaiopsis

Pi 0.0127

MarkerSeek score 53.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1366
variable_sites: 26
pi: 0.0127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	TGGTTGGTCCGTCAGAAAAG	TGGACCAGAGAATGCGATTG	50.0	50.0	58.0	58.0	2337-2348 bp	1.0	80.9
trnT-GGU-psbD_p2	ACGGGGAATACTTTCAGGGA	TGGACCAGAGAATGCGATTG	50.0	50.0	58.0	58.0	2358-2369 bp	1.0	80.8
trnT-GGU-psbD_p3	TGGTTGGTCCGTCAGAAAAG	AAATCCCCTTGTGCTTCAGG	50.0	50.0	58.0	58.1	2192-2203 bp	1.0	80.6
trnT-GGU-psbD_p4	ACGGGGAATACTTTCAGGGA	AAATCCCCTTGTGCTTCAGG	50.0	50.0	58.0	58.1	2213-2224 bp	1.0	80.6
trnT-GGU-psbD_p5	GCTCACACTAAGCTCGGAAA	TGGACCAGAGAATGCGATTG	50.0	50.0	57.9	58.0	2309-2320 bp	1.0	80.5