Hotspot

psbM-trnD-GUC

LSC · 31267-32424 · 1158 bp

Back to Brassaiopsis

Pi 0.0199

MarkerSeek score 75.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 33
pi: 0.0199
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.2	58.5	1084-1290 bp	1.0	68.1
psbM-trnD-GUC_p2	AAGCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.9	58.5	1085-1291 bp	1.0	65.6
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1063-1269 bp	1.0	64.5
psbM-trnD-GUC_p4	AGCAGAAAAGCAGTAGGAACT	GTTCAATTGGTCAGAGCACC	42.9	50.0	57.2	57.0	1085-1291 bp	1.0	63.9
psbM-trnD-GUC_p5	GCAGAAAAGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.7	58.5	1083-1289 bp	1.0	63.7