Hotspot

trnC-GCA-petN

LSC · 29530-30208 · 679 bp

Back to Brassaiopsis

Pi 0.00687

MarkerSeek score 65.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 7
pi: 0.00687
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	CGATATGGCCGAGTGGTAAG	TTAAAGCAGCCCAAGCAAGA	55.0	45.0	57.9	58.0	766-793 bp	1.0	82.8
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	726-753 bp	1.0	82.8
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	720-747 bp	1.0	81.4
trnC-GCA-petN_p4	CGATATGGCCGAGTGGTAAG	CAGCCCAAGCAAGACTTACT	55.0	50.0	57.9	57.8	760-787 bp	1.0	81.3
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	744-771 bp	1.0	80.6