Hotspot

psbI-trnS-GCU

LSC · 9228-9365 · 138 bp

Back to Brassaiopsis

Pi 0.029

MarkerSeek score 66.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 6
pi: 0.029
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGATCCGGGGCGTA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.1	58.0	246 bp	1.0	81.1
psbI-trnS-GCU_p2	TATCTAATGATCCGGGGCGT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.1	58.0	247 bp	1.0	81.1
psbI-trnS-GCU_p3	ATCTAATGATCCGGGGCGTA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.1	58.1	270 bp	1.0	80.4
psbI-trnS-GCU_p4	TATCTAATGATCCGGGGCGT	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.1	58.1	271 bp	1.0	80.4
psbI-trnS-GCU_p5	TCTAATGATCCGGGGCGTAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.3	58.0	245 bp	1.0	79.3