Hotspot

rps16-trnQ-UUG

LSC · 6350-8090 · 1741 bp

Back to Brassaiopsis

Pi 0.0125

MarkerSeek score 66.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1741
variable_sites: 31
pi: 0.0125
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1799-1840 bp	1.0	82.2
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1814-1855 bp	1.0	80.4
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1813-1854 bp	1.0	80.4
rps16-trnQ-UUG_p4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1870-1911 bp	1.0	79.2
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1803-1844 bp	1.0	77.5