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trnK-UUU-rps16

LSC · 4333-5226 · 894 bp

Back to Brassaiopsis

Pi 0.0208

MarkerSeek score 66.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 894
variable_sites: 27
pi: 0.0208
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGAATCCTTCGCTTCATCCA	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.1	58.3	1021-1048 bp	1.0	81.4
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	973-1000 bp	1.0	80.7
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	972-999 bp	1.0	80.7
trnK-UUU-rps16_p4	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	988-1015 bp	1.0	79.9
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	982-1009 bp	1.0	79.7