Hotspot

ycf1

SSC · 123879-129470 · 5592 bp

Back to Brandisia

Pi 0.00987

MarkerSeek score 42.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5592
variable_sites: 228
pi: 0.00987
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCGAGTGTAGGTGGGGAT	AAGAGGGATCCACCGAAGAA	50.0	50.0	58.1	58.0	492-522 bp	1.0	83.0
ycf1_p2	TTTCGAGTGTAGGTGGGGAT	ACAGGAACAAGAGGGATCCA	50.0	50.0	58.1	58.0	500-530 bp	1.0	82.9
ycf1_p3	TAAAGCCAAGGGTTGCAGAG	ACAGGAACAAGAGGGATCCA	50.0	50.0	58.1	58.0	537-567 bp	1.0	82.7
ycf1_p4	TTTCGAGTGTAGGTGGGGAT	GCAGAATACCGTCACCCATT	50.0	50.0	58.1	58.0	624-654 bp	1.0	82.6
ycf1_p5	TAAAGCCAAGGGTTGCAGAG	GCAGAATACCGTCACCCATT	50.0	50.0	58.1	58.0	661-691 bp	1.0	82.4