Hotspot

rpl22

LSC · 83953-84423 · 471 bp

Back to Brandisia

Pi 0.013

MarkerSeek score 58.80

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 471
variable_sites: 23
pi: 0.013
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GTTGTGCAAACCAAAGGGAA	TCCGAGGACATGCAAAAAGT	45.0	45.0	57.3	57.7	625-631 bp	1.0	76.3
rpl22_p2	GTTGTGCAAACCAAAGGGAA	CCGAGGACATGCAAAAAGTG	45.0	50.0	57.3	57.7	624-630 bp	1.0	75.8
rpl22_p3	TGTGCAAACCAAAGGGAATG	TCCGAGGACATGCAAAAAGT	45.0	45.0	57.1	57.7	623-629 bp	1.0	74.5
rpl22_p4	TGTGCAAACCAAAGGGAATG	CCGAGGACATGCAAAAAGTG	45.0	50.0	57.1	57.7	622-628 bp	1.0	74.0
rpl22_p5	CCAAGTCTGAAACCAAGTGGA	TCCGAGGACATGCAAAAAGT	47.6	45.0	58.1	57.7	587-593 bp	1.0	73.3