Hotspot

ndhF-rpl32

SSC · 112859-113322 · 464 bp

Back to Brandisia

Pi 0.0114
MarkerSeek score 52.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
464
variable_sites
20
pi
0.0114
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAACGAAAGGT TGATGCAGAAGTACGTTTTT 50.0 35.0 57.7 53.5 519-561 bp 1.0 48.6
ndhF-rpl32_p2 CTGGAAGTGGAACGAAAGGT TTGATGCAGAAGTACGTTTT 50.0 35.0 57.7 53.5 520-562 bp 1.0 48.6
ndhF-rpl32_p3 CTGGAAGTGGAACGAAAGGT TTTGATGCAGAAGTACGTTT 50.0 35.0 57.7 53.5 521-563 bp 1.0 48.6
ndhF-rpl32_p4 CTGGAAGTGGAACGAAAGGT TTTTGATGCAGAAGTACGTT 50.0 35.0 57.7 53.5 522-564 bp 1.0 48.6
ndhF-rpl32_p5 CTGGAAGTGGAACGAAAGGT TTTTTGATGCAGAAGTACGT 50.0 35.0 57.7 53.5 523-565 bp 1.0 48.6