Hotspot

psbT-psbN

LSC · 75014-75073 · 60 bp

Back to Brandisia

Pi 0.0558

MarkerSeek score 65.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 7
pi: 0.0558
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	47.6	55.0	57.6	57.7	174 bp	1.0	69.8
psbT-psbN_p2	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	126 bp	1.0	69.8
psbT-psbN_p3	CGAGAACCACCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	47.6	50.0	57.6	57.6	182 bp	1.0	68.9
psbT-psbN_p4	CGAGAACCACCTAAAGTTCCA	TAAGTTTTACCGGGTACGCC	47.6	50.0	57.6	57.6	185 bp	1.0	68.8
psbT-psbN_p5	CGAGAACCACCTAAAGTTCCA	CGGGTACGCCTTATATACCG	47.6	55.0	57.6	57.5	175 bp	1.0	67.6