Hotspot

psbB-psbT

LSC · 74713-74905 · 193 bp

Back to Brandisia

Pi 0.0176

MarkerSeek score 57.00

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 11
pi: 0.0176
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TTTTTGCCGGTATTGACCCA	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	58.0	57.6	370-391 bp	1.0	73.0
psbB-psbT_p2	GCCGGTATTGACCCAGATTT	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.9	57.6	365-386 bp	1.0	72.6
psbB-psbT_p3	TTTTTGCCGGTATTGACCCA	GGAACTTTAGGTGGTTCTCGA	45.0	47.6	58.0	57.3	369-390 bp	1.0	70.8
psbB-psbT_p4	TCAGAGATGTTTTTGCCGGT	TGGAACTTTAGGTGGTTCTCG	45.0	47.6	57.7	57.6	379-400 bp	1.0	70.7
psbB-psbT_p5	GCCGGTATTGACCCAGATTT	GGAACTTTAGGTGGTTCTCGA	50.0	47.6	57.9	57.3	364-385 bp	1.0	70.4