Hotspot

psbE-petL

LSC · 65818-66721 · 904 bp

Back to Brandisia

Pi 0.0127

MarkerSeek score 56.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 904
variable_sites: 47
pi: 0.0127
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACGAATAACCAACCTGCA	GTCGTATCTTGCTCAGACCA	45.0	50.0	57.4	57.1	1107-1149 bp	1.0	71.7
psbE-petL_p2	ACGTTCTCCTGTGTTTCCAG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1016-1058 bp	0.909091	70.3
psbE-petL_p3	CGTTCTCCTGTGTTTCCAGA	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.5	57.1	1015-1057 bp	0.909091	68.1
psbE-petL_p4	ACGTTCTCCTGTGTTTCCAG	GTCGTATCTTGCTCAGACCAA	50.0	47.6	57.8	57.8	1016-1058 bp	0.909091	68.0
psbE-petL_p5	ACGTTCTCCTGTGTTTCCAG	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.8	56.8	1017-1059 bp	0.909091	68.0