Hotspot

psaB-psaA

LSC · 39637-39661 · 25 bp

Back to Brandisia

Pi 0.0335

MarkerSeek score 58.80

Primer available yes

Label psaB-psaA

Feature Metadata

label_name: psaB-psaA
feature_type: igs
region: LSC
length_bp: 25
variable_sites: 2
pi: 0.0335
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB-psaA_p1	AACCCTTGGCTAAACCTTGG	GTACAAGGACGTGCTGTAGG	50.0	55.0	58.0	58.0	156 bp	1.0	81.4
psaB-psaA_p2	AACCCTTGGCTAAACCTTGG	ACAACATGGGCGTTCTTCTT	50.0	45.0	58.0	58.0	105 bp	1.0	81.3
psaB-psaA_p3	AACCCTTGGCTAAACCTTGG	TACAAGGACGTGCTGTAGGA	50.0	50.0	58.0	58.1	155 bp	1.0	80.8
psaB-psaA_p4	AACCCTTGGCTAAACCTTGG	GGGCGTTCTTCTTAGCAAGA	50.0	50.0	58.0	57.9	98 bp	1.0	80.8
psaB-psaA_p5	AATACGACGAGTAGTGGGGT	GTACAAGGACGTGCTGTAGG	50.0	55.0	57.9	58.0	184 bp	1.0	80.2