Hotspot

atpA-atpF

LSC · 12078-12159 · 82 bp

Back to Brandisia

Pi 0.0477

MarkerSeek score 68.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 82
variable_sites: 13
pi: 0.0477
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	AATTTCGTCGGCTCGAATGG	TTAGCATGTTGGGAGCGATG	50.0	50.0	59.0	58.3	138-179 bp	1.0	80.4
atpA-atpF_p2	AATTTCGTCGGCTCGAATGG	TGTTGGGAGCGATGAAAGAA	50.0	45.0	59.0	57.4	132-173 bp	1.0	78.9
atpA-atpF_p3	GTCGGCTCGAATGGTTACC	TTAGCATGTTGGGAGCGATG	57.9	50.0	58.3	58.3	132-173 bp	1.0	78.0
atpA-atpF_p4	AATTTCGTCGGCTCGAATGG	ATTAGCATGTTGGGAGCGAT	50.0	45.0	59.0	57.4	139-180 bp	1.0	77.8
atpA-atpF_p5	ATTTCGTCGGCTCGAATGG	TTAGCATGTTGGGAGCGATG	52.6	50.0	58.3	58.3	137-178 bp	1.0	77.8