Hotspot

trnS-GCU-trnG-UCC

LSC · 8760-9441 · 682 bp

Back to Brandisia

Pi 0.00895
MarkerSeek score 47.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
682
variable_sites
17
pi
0.00895
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 751-766 bp 1.0 54.4
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 750-765 bp 1.0 54.4
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 754-769 bp 1.0 53.4
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 753-768 bp 1.0 53.4
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 752-767 bp 1.0 53.4