Hotspot

ycf1

SSC · 112450-118077 · 5628 bp

Pi 0.169

MarkerSeek score 76.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5628
variable_sites: 1681
pi: 0.169
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGATCCATGAATTGGGCAA	CACTGCCACCGCATAGTAAT	45.0	50.0	57.6	58.0	871-898 bp	0.5	59.4
ycf1_p2	TTCGATCCATGAATTGGGCA	CACTGCCACCGCATAGTAAT	45.0	50.0	57.6	58.0	872-899 bp	0.5	59.4
ycf1_p3	TCGATCCATGAATTGGGCAA	TCAATGAATCCACTGCCACC	45.0	50.0	57.6	58.2	881-908 bp	0.5	58.5
ycf1_p4	TTCGATCCATGAATTGGGCA	TCAATGAATCCACTGCCACC	45.0	50.0	57.6	58.2	882-909 bp	0.5	58.5
ycf1_p5	TCGATCCATGAATTGGGCAA	TCGTCAATGAATCCACTGCC	45.0	50.0	57.6	58.3	884-911 bp	0.5	57.7