Hotspot

ndhH

SSC · 118846-120027 · 1182 bp

Pi 0.204

MarkerSeek score 69.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 423
pi: 0.204
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CAGTTGGATATTCGGGAGCC	CCAGCATATTCAGGCCCAAT	55.0	50.0	58.1	58.0	1524-1526 bp	0.5	59.3
ndhH_p2	GGCTCCTGGCTTATTTGTCA	CCAGCATATTCAGGCCCAAT	50.0	50.0	57.9	58.0	1580-1582 bp	0.5	59.2
ndhH_p3	CAGTTGGATATTCGGGAGCC	CCAACGGACCAGCATATTCA	55.0	50.0	58.1	58.0	1532-1534 bp	0.5	59.0
ndhH_p4	GGCTCCTGGCTTATTTGTCA	CCAACGGACCAGCATATTCA	50.0	50.0	57.9	58.0	1588-1590 bp	0.5	58.9
ndhH_p5	CAGTTGGATATTCGGGAGCC	TGTTCCAACGAATCACACGT	55.0	45.0	58.1	58.1	1892-1894 bp	0.5	58.8