Hotspot

ndhF

SSC · 129191-131428 · 2238 bp

Back to Brahea

Pi 0.197
MarkerSeek score 70.20
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2238
variable_sites
779
pi
0.197
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.78
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 CAGTTCTTCGGGCTATGTCA CGGTTTGAAAATGCTGTACGA 50.0 42.9 57.3 57.5 2939-2967 bp 0.5 42.9
ndhF_p2 CAGTTCTTCGGGCTATGTCA TCGGTTTGAAAATGCTGTACG 50.0 42.9 57.3 57.5 2940-2968 bp 0.5 42.9
ndhF_p3 TCAGTTCTTCGGGCTATGTC CGGTTTGAAAATGCTGTACGA 50.0 42.9 57.0 57.5 2940-2968 bp 0.5 40.6
ndhF_p4 TCAGTTCTTCGGGCTATGTC TCGGTTTGAAAATGCTGTACG 50.0 42.9 57.0 57.5 2941-2969 bp 0.5 40.6
ndhF_p5 CAGTTCTTCGGGCTATGTCAA CGGTTTGAAAATGCTGTACGA 47.6 42.9 58.0 57.5 2939-2967 bp 0.5 40.1