Hotspot

ndhD

SSC · 125032-126537 · 1506 bp

Pi 0.236

MarkerSeek score 72.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 621
pi: 0.236
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GACAAAAGCCCGTACTCGAT	CTTATTGGAGTTGGGACCCG	50.0	55.0	58.0	58.0	2013 bp	0.5	59.8
ndhD_p2	GACAAAAGCCCGTACTCGAT	ATGCAGCTCTTTTATGCGGA	50.0	45.0	58.0	58.0	2322 bp	0.5	59.7
ndhD_p3	GACAAAAGCCCGTACTCGAT	TGCAGCTCTTTTATGCGGAT	50.0	45.0	58.0	58.0	2321 bp	0.5	59.7
ndhD_p4	CTGCTTCGTCTGGTGTCTAC	CTTATTGGAGTTGGGACCCG	55.0	55.0	58.0	58.0	2410 bp	0.5	59.7
ndhD_p5	CTGCTTCGTCTGGTGTCTAC	TGCAGCTCTTTTATGCGGAT	55.0	45.0	58.0	58.0	2718 bp	0.5	59.6