Hotspot

ndhA

SSC · 120029-122171 · 2143 bp

Pi 0.209

MarkerSeek score 68.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2143
variable_sites: 792
pi: 0.209
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTCAGAGTAGAAGCCCCAA	CGGGCATACACGAACACATA	50.0	50.0	58.0	58.1	2659-2660 bp	0.5	59.2
ndhA_p2	ACTTTCCCCTGGAGATGGAA	CGGGCATACACGAACACATA	50.0	50.0	57.9	58.1	2601-2602 bp	0.5	59.0
ndhA_p3	TGGGAAGTCCAATGGCAAAA	CGGGCATACACGAACACATA	45.0	50.0	57.9	58.1	2901-2902 bp	0.5	58.7
ndhA_p4	GGGCATTATTGGTGGAGAGG	ACCTTGCAGCTCGTATTGTT	55.0	45.0	58.0	57.8	2858-2859 bp	0.5	58.5
ndhA_p5	TGTCAGAGTAGAAGCCCCAA	ACCTTGCAGCTCGTATTGTT	50.0	45.0	58.0	57.8	2495-2496 bp	0.5	58.3