Hotspot

ccsA

SSC · 126754-127719 · 966 bp

Back to Brahea

Pi 0.229
MarkerSeek score 70.70
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
966
variable_sites
390
pi
0.229
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.83
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TTCTCGATGGCTTCTCTTGC GTGAAATCGGTAGACACGCT 50.0 50.0 58.0 58.0 1693 bp 0.5 60.0
ccsA_p2 TTCTCGATGGCTTCTCTTGC TGAAATCGGTAGACACGCTG 50.0 50.0 58.0 58.0 1692 bp 0.5 60.0
ccsA_p3 CTCGATGGCTTCTCTTGCAT GTGAAATCGGTAGACACGCT 50.0 50.0 58.0 58.0 1691 bp 0.5 59.7
ccsA_p4 CTCGATGGCTTCTCTTGCAT TGAAATCGGTAGACACGCTG 50.0 50.0 58.0 58.0 1690 bp 0.5 59.7
ccsA_p5 CGACATGGGACTCAATGGAG GTGAAATCGGTAGACACGCT 55.0 50.0 58.1 58.0 1862 bp 0.5 59.7