Hotspot

rpl32-ndhF

SSC · 128836-129190 · 355 bp

Back to Brahea

Pi 0.185
MarkerSeek score 61.60
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
355
variable_sites
117
pi
0.185
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.78
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 CAGTTCTTCGGGCTATGTCA AATCGGTCGTGTGAACCAAA 50.0 45.0 57.3 58.0 1064-1083 bp 0.5 55.1
rpl32-ndhF_p2 CAGTTCTTCGGGCTATGTCA ATCGGTCGTGTGAACCAAAA 50.0 45.0 57.3 58.0 1063-1082 bp 0.5 55.1
rpl32-ndhF_p3 CAGTTCTTCGGGCTATGTCA CATACGTGGAGGGGGAATTG 50.0 55.0 57.3 58.0 1300-1319 bp 0.5 55.0
rpl32-ndhF_p4 CAGTTCTTCGGGCTATGTCA AGCAACTGCACCGACAAATA 50.0 45.0 57.3 58.1 1271-1290 bp 0.5 54.4
rpl32-ndhF_p5 CAGTTCTTCGGGCTATGTCA TAGCAACTGCACCGACAAAT 50.0 45.0 57.3 58.1 1272-1291 bp 0.5 54.4